The present invention relates to the field of immunology, and discloses a time resolved fluoro-immunochromatography method for quantitatively detecting beta-adrenoceptor agonists in an animal. The time resolved fluoro-immunochromatography method mainly comprises: (1) obtaining Eu<3+>-labeled goat anti-mouse IgG; (2) obtaining Sm<3+> labeled goat anti-rabbit IgG; (3) preparing a reference standard substance; (4) preparing a solid phase coated plate; and (5) taking the coated plate obtained in the step (4), adding 50 [mu]l of the standard substance obtained in the step (3) or a treated sample, adding 100 [mu]l of the label obtained in the step (1) and 100 [mu]l of the label obtained in the step (2), carrying out oscillation incubation for 30 min at a room temperature, washing five times with a 0.05 mol/L Tris-HCl washing liquid having the pH value of 7.4 and containing 0.9% of NaCl and 0.4-0.6% of methanol, adding 200 [mu]l of a reinforcing liquid, carrying out oscillation incubation for 10 min, and detecting with a semi-automatic fluorescence detector. The time resolved fluoro-immunochromatography method of the present invention has advantages of excellent stability, high sensitivity, and good reproducibility.