The invention discloses a laparoscope puncture sheath. The laparoscope puncture sheath includes an outer pipe and an inner cylinder which is sleeved in the outer pipe, the longitudinal section of the inner cylinder is in a T shape, the two sides of the outer wall of the upper end of the inner cylinder are separately provided an upper clamping groove and a lower clamping groove, a circular ring is fixed at the lower end of the inner cylinder, three or more supports wings are evenly distributed on the circular ring, joints between the support wings and the circular ring are all provided with torsion springs, the longitudinal section of the outer pipe is also in a T shape, two through holes are formed in the two sides of the pipe wall of the upper end of the outer pipe respectively, retractable mechanisms are arranged in the through holes, and the retractable mechanisms can fix the clamping grooves of the inner cylinder and adjust the relative position between the inner cylinder and the outer pipe. According to the puncture sheath, through stretching motions of the support wings in the abdominal cavity, the fixation of the puncture sheath in the abdominal cavity is achieved, the problem can be effectively solved that current puncture sheaths are not easy to fix, and patients' injuries caused by operations are greatly reduced; meanwhile, the laparoscope puncture sheath is relatively good in air tightness, provides stable and reliable operation channels for the operations and had relatively high practical value.