The invention provides a biological membrane clamp and belongs to the technical field of foreign substance stimulation detection. The biological membrane clamp comprises a clamping part, light-transmitting parts, a holding part, a sealing part and a reagent adding part, wherein the clamping part is formed by jointing of front and rear clamping components which; the sealing part is arranged at a joint end of the rear clamping component; front and rear light-transmitting parts are respectively arranged at non-joint ends of the corresponding front and rear clamping components; the clamping part has a hollow structure; the clamping part, the sealing part and the light-transmitting parts are formed into a chamber; the reagent adding part is arranged on the upper part of the clamping part; and the holding part is arranged at the top of the front clamping component. The biological membrane can be conveniently and quickly clamped for performing a stimulation detection experiment for foreign substances, such as, chemicals, cosmetics and drugs, the experiment efficiency can be increased and the animal experiment can be avoided.