基本信息:
- 专利标题： 多靶点联合的CAR‑T细胞制剂与制备方法
- 专利标题（英）：Multi-target combined CAR (chimeric antigen receptor)-T cell preparation and preparation method
- 申请号：CN201611158116.3 申请日：2016-12-15
- 公开(公告)号：CN106822184A 公开(公告)日：2017-06-13
- 发明人： 梁爱斌 , 刘劼 , 孙毅 , 张文君 , 汪俊帮 , 李萍 , 唐晓晨 , 陈彩琴 , 朱益辉
- 申请人： 梁爱斌 , 刘劼 , 孙毅 , 张文君 , 汪俊帮 , 李萍 , 唐晓晨 , 陈彩琴 , 朱益辉
- 申请人地址： 上海市普陀区新村路389弄2号楼7楼; ; ; ; ; ; ; ;
- 专利权人： 梁爱斌,刘劼,孙毅,张文君,汪俊帮,李萍,唐晓晨,陈彩琴,朱益辉
- 当前专利权人： 梁爱斌,刘劼,孙毅,张文君,汪俊帮,李萍,唐晓晨,陈彩琴,朱益辉
- 当前专利权人地址： 上海市普陀区新村路389弄2号楼7楼; ; ; ; ; ; ; ;
- 代理机构： 上海三方专利事务所
- 代理人： 吴玮; 钱品兴
- 主分类号： A61K35/17
- IPC分类号： A61K35/17 ; C12N15/86 ; C12N15/12 ; C12N5/10 ; A61P35/00 ; A61P35/02
The present invention relates to a CAR (chimeric antigen receptor)-T cell preparation and a preparation method. The preparation method comprises the steps: i) designing a combination of multiple tumor antigens by using genetic engineering techniques, wherein the combination of tumor antigens comprises a) CD19 and b) one or two of CD20, CD138 and CD123); ii) inserting the combination of tumor antigens between the positions of 4513 and 4514 of a vector sequence to form a CAR, wherein the base sequence of the vector sequence is shown in SEQ ID NO. 1; iii) performing virus packaging on the CAR; iv) transfecting the CAR into T cells by viruses; v) culturing by using a culture medium; vi) mixing the prepared CAR-T cells with human serum albumin and normal saline, wherein the concentration of CAR-T cells is 0.5-1*10<6> cells/ml, the positive rate of the CAR is 25% or more, and the motility rate is 95% or more. Compared with the prior art, the preparation and preparation method disclosed by the invention have the advantages that the recognition ability for tumor cells can be improved and the killing efficiency of T cells to the tumor cells is ensured by establishing CAR-T cells of a combination of a plurality of different tumor antigens and multi-target recognition.
IPC结构图谱:
| A | 人类生活必需 |
| --A61 | 医学或兽医学；卫生学 |
| ----A61K | 医用、牙科用或梳妆用的配制品 |
| ------A61K35/00 | 含有其有不明结构的原材料或其反应产物的医用配制品 |
| --------A61K35/02 | .来源于非生命物质的 |
| ----------A61K35/14 | ..血液 |
| ------------A61K35/17 | ...淋巴细胞；B细胞；T细胞；自然杀伤细胞；干扰素激活或细胞因子激活的淋巴细胞 |