Relating to the technical field of laminated glass, the invention discloses a preparation method of a blue ray-eliminating heat insulation and anti-ultraviolet membrane. The method includes: dispersing a nano material with selective permeability to sunlight evenly into polyester resin with good bonding function to glass, and coating a PET film with the mixture uniformly, performing drying to form a membrane, attaching a release film and performing rolling molding. The preparation method of the blue ray-eliminating heat insulation and anti-ultraviolet membrane provided by the invention employs supergravity process to prepare Al-doped ZnO nanoparticles so as to absorb blue ray and caesium, Ti, W doped SnO nanoparticles mainly for obstructing near infrared rays. A variety of inorganic nano oxide particles are adopted as the heat insulation medium, the performance is stable, the service life is long, and nano-scale dispersion does not affect lighting. The film prepared by the method can be pasted on glass, and has the characteristics of heat insulation, ultraviolet prevention, elimination of excess blue ray to reduce the damage to eyes, and good transparency.