基本信息:
- 专利标题： 用于快速检测ABCB1基因C3435T多态性的核酸、试剂盒及方法
- 专利标题（英）：Nucleic acid, kit and method for rapid detection of C3435T polymorphism of ABCB1 gene
- 申请号：CN201611100812.9 申请日：2016-12-05
- 公开(公告)号：CN106755352A 公开(公告)日：2017-05-31
- 发明人： 杨惠娟 , 邹芳 , 段卫涛 , 赵平锋
- 申请人： 武汉海吉力生物科技有限公司
- 申请人地址： 湖北省武汉市东湖技术开发区高新大道858号生物医药园二期A82-1栋
- 专利权人： 武汉海吉力生物科技有限公司
- 当前专利权人： 武汉海吉力生物科技有限公司
- 当前专利权人地址： 湖北省武汉市东湖技术开发区高新大道858号生物医药园二期A82-1栋
- 代理机构： 北京汇泽知识产权代理有限公司
- 代理人： 程殿军; 张瑾
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12N15/11
The invention discloses a nucleic acid, kit and method for rapid detection of C3435T polymorphism of an ABCB1 gene. The nucleic acid comprises detection primers and a detection probe, wherein the detection primers comprise a wild downstream primer, a mutant downstream primer and a common upstream primer; a nucleotide sequence of the wild downstream primer is as shown in SEQ ID No.1, a nucleotide sequence of the mutant downstream primer is as shown in SEQ ID No.2, a nucleotide sequence of the common upstream primer is as shown in SEQ ID No.3, and a nucleotide sequence of the detection probe is as shown in SEQ ID No.4. The kit and method for real-time fluorescent quantitative PCR rapid detection of C3435T polymorphism of the ABCB1 gene have the advantages of being convenient to use, simple to operate and high in automatic degree, greatly simplifying the operation process and reducing pollution in the operation process, and the detection effect is good; the kit has the characteristics of high sensitivity, specificity, accuracy and precision.
公开/授权文献:
- CN106755352B 用于快速检测ABCB1基因C3435T多态性的核酸、试剂盒及方法 公开/授权日：2020-04-07