基本信息:
- 专利标题： 同步检测肺炎克雷伯氏菌和豚鼠气单胞菌的双重PCR引物及其检测方法
- 专利标题（英）：Double PCR (Polymerase chain reaction) primer for simultaneous detection of Klebsiella pneumoniae and Aeromonas caviae and detection method of double PCR primer
- 申请号：CN201710089902.0 申请日：2017-02-20
- 公开(公告)号：CN106701994A 公开(公告)日：2017-05-24
- 发明人： 滕涛 , 习丙文 , 梁利国 , 陈凯 , 潘良坤 , 谢骏 , 徐跑
- 申请人： 中国水产科学研究院淡水渔业研究中心
- 申请人地址： 江苏省无锡市滨湖区滨湖街道山水东路9号
- 专利权人： 中国水产科学研究院淡水渔业研究中心
- 当前专利权人： 中国水产科学研究院淡水渔业研究中心
- 当前专利权人地址： 江苏省无锡市滨湖区滨湖街道山水东路9号
- 代理机构： 无锡市大为专利商标事务所
- 代理人： 殷红梅; 张仕婷
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12Q1/04 ; C12N15/11
The invention relates to a double PCR (Polymerase chain reaction) primer for simultaneous detection of Klebsiella pneumoniae and Aeromonas caviae and a detection method of the double PCR primer, and belongs to the technical field of biological detection. The Klebsiella pneumoniae and Aeromonas caviae can be simultaneously detected in the same reaction system by taking a Klebsiella pneumoniae ompC gene and an Aeromonas caviae ahal gene as detection genes, the sizes of amplified products are 418bp and 213bp respectively, and the detection sensitivity of the two pairs of primers is 1.0*10<3>pg/mu L and 3.3*10<2>pg/mu L respectively. The double PCR detection method provided by the invention has the advantages of high specificity, high sensitivity, simple operation and short time, and can be used for simultaneous detection of Klebsiella pneumoniae and Aeromonas caviae.