
基本信息:
- 专利标题: 一种荧光探针检测血吸虫DNA的方法
- 专利标题(英):Method for detecting DNA of schistosoma through fluorescent probe
- 申请号:CN201611206727.0 申请日:2016-12-23
- 公开(公告)号:CN106701940A 公开(公告)日:2017-05-24
- 发明人: 赵松 , 杨坤 , 李伟 , 张键锋 , 羊海涛 , 郭利川 , 刘小曼 , 王智宏 , 应清界
- 申请人: 江苏省血吸虫病防治研究所 , 江苏奇天基因生物科技有限公司
- 申请人地址: 江苏省无锡市滨湖区梅园杨巷117号;
- 专利权人: 江苏省血吸虫病防治研究所,江苏奇天基因生物科技有限公司
- 当前专利权人: 江苏省血吸虫病防治研究所,江苏奇天基因生物科技有限公司
- 当前专利权人地址: 江苏省无锡市滨湖区梅园杨巷117号;
- 代理机构: 北京高沃律师事务所
- 代理人: 王加贵
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68
The invention provides a method for detecting DNA of schistosoma through a fluorescent probe. An amplification system and the DNA of the schistosoma are mixed, isothermal amplification is carried out to obtain an amplified product, and the amplified product is subjected to real-time detection through a fluorescent probe method. The amplification system comprises the components of a Tris buffer solution, potassium acetate, magnesium acetate, dithiothreitol, polyethylene glycol, ATP, dNTPs, phosphocreatine single-strand binding protein, recombinase, UvsY protein, exonuclease, the fluorescent probe, DNA polymerase and a schistosoma DNA specific primer. The temperature of isothermal amplification is 35-45 DEG C. The time of isothermal amplification is 5-20 min. The method does not rely on a thermal cycler, operation is easy, the DNA of the schistosoma can be detected at the low temperature (35-45 DEG C), the time used for amplification is short (5-20 min), and the method is suitable for primary and on-site detection.
公开/授权文献:
- CN106701940B 一种荧光探针检测血吸虫DNA的方法 公开/授权日:2021-08-24