The invention discloses a temperature-sensitive gel for a skin injury and a preparation method of the temperature-sensitive gel and relates to the technical field of skin repair administration. The temperature-sensitive gel is prepared from the following raw materials in parts by weight: 5-20 parts of poloxamer 407, 0.01-0.1 part of a bacteriostatic agent, 0.001-0.05 part of a human epidermal growth factor, 0.2-1.0 part of a humectant, 2-10 parts of sodium hyaluronate and the balance of water for injection water. The temperature-sensitive gel is prepared from the poloxamer 407 as a main raw material, and exists in a solution form at a low temperature; administration is convenient; the temperature-sensitive gel is converted into a gel state at a high temperature and is smooth and soft in surface, free of thrill to the administration part, free of foreign body sensation and good in biocompatibility; the sodium hyaluronate is very high in biocompatibility, beneficial to long-term moisture retention of the skin and beneficial to tissue cell regeneration and is capable of compacting the skin and preventing dehydration; and the human epidermal growth factor has an acceleration effect on mucosa repairing of the skin injury, so that the wound healing cycle is shortened to meet the structure design and clinical requirements.