The invention discloses a DHAV (Duck Hepatitis A Virus) III type complex live vaccine and a preparation method thereof. The method firstly discloses the DHAV III type complex live vaccine, which comprises antigen-antibody complex and pharmaceutically acceptable freeze-drying protective agents, wherein the antigen-antibody complex is prepared from DHAV III type chick embryo weakening strain JS57 virus liquid and duck anti-DHAV III type egg yolk antibodies. The invention further discloses a preparation method of the DHAV III type complex live vaccine. The preparation method comprises the following steps of mixing the freeze-drying protective agents with the antigen-antibody complex prepared from the DHAV III type chick embryo weakening strain JS57 virus liquid and the duck anti-DHAV III type egg yolk antibodies; performing freeze vacuum drying; and obtaining the DHAV III type complex live vaccine. The preparation method provided by the invention has the advantages that the antibody obtaining path is simple; the production cost is low; the prepared DHAV III type complex live vaccine can break through the maternal antibody interference to generate high neutralizing antibody valence; and the production rate on ducklings is improved.