The invention provides medicine target protein of serums after a Yimusake tablet acts on rats with an abnormal balham syndrome and an abnormal Balham type erectile dysfunction disease, and a penis smooth muscle tissue, and a screening method thereof. The method comprises the following steps of 1, providing a normal comparison set, a syndrome set, a syndrome medicine set, a disease set and a disease medicine set; 2, collecting whole blood, separating the serum and the penis smooth muscle tissue from the whole blood; 3, extracting the protein; 4, purifying after marking; 5, acquiring a fingerprint spectrum after spectrum detection, analyzing protein compositions after screening, and acquiring each set of serums and the penis smooth muscle tissue protein; and 6, acquiring a Yimusake tablet action target protein system by making statistics, comparing and screening. According to the protein and the screening method of the protein provided by the invention, the action target of the uigur medicine, the Yimusake tablet, on the abnormal Balham type erectile dysfunction in uigur medicine is clear, and a foundation is laid for further disclosure of the action mechanism of the Yimusake tablet and new and secondary development of the Yimusake tablet.