The invention discloses an ordered extralarge-hole-pitch alumina film and a preparation method thereof. The alumina film has high hardness and elastic modulus, low reflectivity and obvious photoluminescence performance. The surface of the film is composed of innumerable holes. The bottoms of the holes are distributed in ordered hexagons. According to the ordered extralarge-hole-pitch alumina film and the preparation method thereof, macromolecular organic acid serves as an electrolyte, the electrolyte and an aluminum base are effectively cooled simultaneously, and the aluminum base is stably oxidized under extra-high voltage. The alumina film can serve as an ideal template for preparing various ordered major-cycle nano array structures of metal, polymer and the like, and can also be used for developing three-dimensional channel alumina films of micro and nano composite structures. The major-cycle alumina film is an excellent bioactivator carrier and has wide application prospects in the aspects of biosensors, medicine transport platforms, solar cells and the like.