基本信息:
- 专利标题： 一种异化Fe(Ⅲ)还原混合菌培养方法
- 专利标题（英）：Dissimilatory Fe (III) reduction mixed bacteria culture method
- 申请号：CN201610936980.5 申请日：2016-10-25
- 公开(公告)号：CN106544273A 公开(公告)日：2017-03-29
- 发明人： 何清明 , 李廷友 , 周国海
- 申请人： 泰州学院
- 申请人地址： 江苏省泰州市海陵区济川东路93号
- 专利权人： 泰州学院
- 当前专利权人： 泰州学院
- 当前专利权人地址： 江苏省泰州市海陵区济川东路93号
- 代理机构： 泰州地益专利事务所
- 代理人： 王楚云
- 主分类号： C12N1/00
- IPC分类号： C12N1/00 ; C02F3/34 ; C02F101/20 ; C02F103/20
The invention discloses a dissimilatory Fe (III) reduction mixed bacteria culture method which comprises the following steps: firstly, taking 5 mL of fresh fecal sewage, putting into a bottle having addition of 50 mL of a culture medium A after sterilization, and shaking evenly for 1-2 minutes, then introducing N2 into the bottle having faeces and the culture medium A to remove O2 in the bottle, covering tightly and sealing with a plug, placing at a 30 DEG C anaerobic constant temperature box in a dark place, and incubating and culturing for 3 days. The method can relatively intuitively observe the culture process of bacteria through a precipitation process, further studies and analyzes the activity and metabolic characteristics of the cultured mixed bacteria, and is relatively simple and convenient.