The invention aims to provide a test method for a rabbit body effect of a hog cholera virus genetic engineering subunit vaccine. The method comprises the following steps: immunizing a to-be-tested rabbit with the hog cholera virus genetic engineering subunit vaccine for 21 days, and then detecting the hog cholera virus antibody level through ELISA (Enzyme Linked Immuno Sorbent Assay) or adopting hog cholera lapinized virus vaccine injection for counteracting toxic substances, and confirming as qualified if at least 4/5 of hog cholera virus antibody block rate S/P of the immunized rabbit is more than or equal to 60% or 4/5 rabbit body is free from typical thermal reaction after the process of counteracting toxic substances. According to the test method for the rabbit body effect of vaccine, provided by the invention, the protecting effect of the hog cholera virus genetic engineering subunit vaccine can be tested, the effect of the hog cholera virus genetic engineering subunit vaccine can be more stably, simply, conveniently and efficiently evaluated with saved time and cost, the defects of the prior art can be overcome, the test method has a positive evaluation guiding function for later mass production and culture process optimization, the test method is more beneficial to the guidance of the clinical application of the hog cholera virus genetic engineering subunit vaccine products and the test method has a wide market application prospect.