The invention relates to an EGFR gene mutation detection primer probe and a kit thereof. The kit comprises a digital PCR master mix used for preparing digital PCR reaction liquor, a liquid droplet stabilizer and primer probe mixed liquor. The primer probe mixed liquor comprises an upstream primer a, a downstream primer a, a deficiency probe a and a detection probe a, wherein the upstream primer a, the downstream primer a, the deficiency probe a and the detection probe a are used for detecting 19 exon E19-Dels locus; the primer probe mixed liquor further comprises a mutation upstream primer b, a mutation probe b, a wild upstream primer b, a wild probe b, and an universal downstream primer b, wherein the mutation upstream primer b, the mutation probe b, the wild upstream primer b, the wild probe b and the universal downstream primer b are used for detecting a 20 exon T790M locus; the primer probe mixed liquor further comprises a mutation upstream primer c, a mutation probe c, a wild upstream primer c, a wild probe c and an universal downstream primer c, wherein the mutation upstream primer c, the mutation probe c, the wild upstream primer c, the wild probe c and the universal downstream primer c are used for detecting 21 exon L858R locus. The EGFR gene mutation detection kit is capable of quickly, conveniently and accurately detecting situations of EGFR gene mutation in a patient with non-small cell lung cancer.