基本信息:
- 专利标题： 一种萨能奶山羊胎儿成纤维细胞miRNA的筛选与鉴定方法
- 专利标题（英）：Screening and identifying method of miRNAs (micro Ribonucleic Acids) of fetal fibroblasts of Saanen dairy goats
- 申请号：CN201610954711.1 申请日：2016-10-27
- 公开(公告)号：CN106520758A 公开(公告)日：2017-03-22
- 发明人： 常卫华 , 武军元 , 王娟红 , 贺建忠 , 王永 , 陈宏伟
- 申请人： 塔里木大学
- 申请人地址： 新疆维吾尔自治区阿拉尔市虹桥南路705号塔里木大学
- 专利权人： 塔里木大学
- 当前专利权人： 塔里木大学
- 当前专利权人地址： 新疆维吾尔自治区阿拉尔市虹桥南路705号塔里木大学
- 代理机构： 北京方圆嘉禾知识产权代理有限公司
- 代理人： 董芙蓉
- 主分类号： C12N15/10
- IPC分类号： C12N15/10 ; C40B50/06 ; C12Q1/68
The invention discloses a screening and identifying method of miRNAs (micro Ribonucleic Acids) of fetal fibroblasts of Saanen dairy goats. The method comprises the following steps: obtaining the fetal fibroblasts of the Saanen dairy goats; extracting RNAs; constructing a library and carrying out high-throughput sequencing; and carrying out data processing. By adopting the high-throughput sequencing, 16395039total_reads are obtained, and redundant data is taken out to obtain clean_reads 16150181 containing Unique sRNAS 205857. Bioinformatic analysis is carried out to obtain 44 known miRNAs and 247 candidate new miRNAs. The quantity of miRNA target genes is 5401, and the quantity of sites of the target genes is 6069; and the quantity of candidate miRNA target genes is 8401, and the quantity of sites of the target genes is 10832.