The invention discloses a liquid crystal compound containing 4-(biphenyl acetenyl)-1,8 naphthalimide, a preparing method and application. Solubility of molecules in mixed liquid crystal is improved by the compound, the linear structure of the molecules is enhanced, and the dichroism of the liquid crystal compound is improved; a push-drawing electron system is formed through high-delocalization electrons on a benzene ring and electron-withdrawing capacity of bisamide bonds. The maximum emission wavelength of the liquid crystal compound in dichloromethane is 502 nm, the liquid crystal compound emits glaucous fluorescence, the fluorescence quantum yield is 0.88, high dichroic ratio and order parameters are represented in liquid crystal, and the liquid crystal compound serving as guest liquid crystal dye can be used for manufacturing a liquid crystal display product particularly a guest-host type liquid crystal displayer. The liquid crystal compound has a typical nematic phase within the temperature range of 160-250 DEG C, and birefringence of a liquid crystal body can be improved by adding the liquid crystal compound into smectic phase liquid crystal, or nematic phase liquid crystal or cholesteric phase liquid crystal.