基本信息:
- 专利标题： 用于盖塔病毒实时荧光定量PCR检测体系及检测方法
- 专利标题（英）：Real-time fluorescence quantification PCR detection system and method for Getah virus
- 申请号：CN201610854388.0 申请日：2016-09-27
- 公开(公告)号：CN106148572A 公开(公告)日：2016-11-23
- 发明人： 史宁 , 闫喜军 , 鲁荣光 , 吕爽 , 胡博 , 张蕾 , 张海玲 , 白雪 , 邓效禹
- 申请人： 中国农业科学院特产研究所
- 申请人地址： 吉林省长春市净月经济开发区聚业大街4899号
- 专利权人： 中国农业科学院特产研究所
- 当前专利权人： 中国农业科学院特产研究所
- 当前专利权人地址： 吉林省长春市净月经济开发区聚业大街4899号
- 代理机构： 北京超凡志成知识产权代理事务所
- 代理人： 王玉桂
- 主分类号： C12Q1/70
- IPC分类号： C12Q1/70 ; C12Q1/68
The invention provides a real-time fluorescence quantification PCR detection system for Getah virus. The detection system comprises a primer group and a probe, wherein the primer group is used for amplifying a gene sequence of the Getah virus, the probe is used for capturing the gene sequence of the Getah virus, the primer group comprises an upstream primer with a base sequence represented by SEQ ID No.1 as well as a downstream primer with a base sequence represented by SEQ ID No.2, and the probe has a base sequence represented by SEQ ID No.3. The primer group and the probe have strong specificities, good repeatability and high sensitivities, the minimum detection template amount can reach 2.03*10<1>copies/microliter, but the minimum detection template amount of a common RT-PCR method only can reach 2.03*10<5>copies/microliter, and the sensitivity of the detection system can reach 10000 times of that of a conventional RT-PCR technique.