基本信息:
- 专利标题： 使用软刻芯片改变结晶基底粗糙度用于蛋白质结晶的方法
- 专利标题（英）：Method for changing roughness of crystalline basement by using soft lithography chip for protein crystallization
- 申请号：CN201610748748.9 申请日：2016-08-29
- 公开(公告)号：CN106117307A 公开(公告)日：2016-11-16
- 发明人： 张辰艳 , 杨雪舟 , 尹大川 , 董晨 , 闫二开
- 申请人： 西北工业大学
- 申请人地址： 陕西省西安市友谊西路127号
- 专利权人： 西北工业大学
- 当前专利权人： 西北工业大学
- 当前专利权人地址： 陕西省西安市友谊西路127号
- 代理机构： 西北工业大学专利中心
- 代理人： 顾潮琪
- 主分类号： C07K1/30
- IPC分类号： C07K1/30 ; G03F7/00
The invention provides a method for changing the roughness of a crystalline basement by using a soft lithography chip for protein crystallization. The method comprises the following steps: firstly, designing and producing a PDMS (Polydimethylsiloxane) chip meeting the crystallization requirements of protein; secondly, dropwise adding a protein crystallization solution on the surface of the PDMS chip, standing and crystallizing. According to the method provided by the invention, by designing the PDMS chip, a controllable crystallization interface with uniform roughness is realized, so that the nucleation probability of the protein on the surface of the basement is improved, and further the aim of improving the success rate of protein crystallization is achieved. According to the method, the screening success rate of protein crystallization conditions can be improved by 1 to 5 times.
公开/授权文献:
- CN106117307B 使用软刻芯片改变结晶基底粗糙度用于蛋白质结晶的方法 公开/授权日：2019-09-24