基本信息:
- 专利标题： 一种非病毒iPSCs诱导方法及其诱导组合物、试剂盒及其获得的iPSCs
- 专利标题（英）：Non-viral iPSCs inducing method, inducing composition and kit thereof and iPSCs obtained by inducing method
- 申请号：CN201610411378.X 申请日：2016-06-13
- 公开(公告)号：CN106086071A 公开(公告)日：2016-11-09
- 发明人： 王淋立 , 陈月花 , 宋立兵 , 莫健
- 申请人： 广州市搏克生物技术有限公司 , 王淋立
- 申请人地址： 广东省广州市高新技术产业开发区科学城掬泉路3号广州国际企业孵化器D区D217号房间
- 专利权人： 广州市搏克生物技术有限公司,王淋立
- 当前专利权人： 未来智人再生医学研究院(广州)有限公司
- 当前专利权人地址： 广东省广州市高新技术产业开发区科学城掬泉路3号广州国际企业孵化器D区D217号房间
- 代理机构： 广州市越秀区哲力专利商标事务所
- 代理人： 赵赛; 马簪
- 主分类号： C12N15/85
- IPC分类号： C12N15/85 ; C12N5/10
The invention discloses a non-viral iPSCs inducing method, an inducing ccomposition and a kit thereof and iPSCs obtained by the inducing method. Specifically, the inducing method comprises the following steps: 1) constructing DNA sequences of expression reprogramming factors POU5F1, SOX2, GLIS1, KLF4, MYCL and hsa-miR-302s into episomal plasmid, so that recombinant plasmid is obtained; and (2) transferring the recombinant plasmid obtained in the step 1) into human somatic cells, and conducing inducing culture, so that the iPSCs is obtained. The method, instead of adding high-risk reprogramming factors, such as c-MYC, SV40-LT, TP53 inhibiting factors and the like, makes use of high-safety reprogramming factor combination, so that the clinical application risk of the iPSCs is reduced; and the method is relatively high in applicability.
公开/授权文献:
- CN106086071B 一种非病毒iPSCs诱导方法及其诱导组合物、试剂盒及其获得的iPSCs 公开/授权日：2019-08-09