The invention relates to a hemibagrus fish interspecific hybridization method and belongs to the technical field of aquatic genetic breeding. Hemibagrus pluriradiatus serves as a male parent, hemibagrus nemurus serves as a female parent, and secondary oxytocic injection, primary LRH-A2 injection and secondary LRH-A2+DOM are conducted on hemibagrus nemurus female fishes; LRH-A2+DOM is injected to hemibagrus pluriradiatus male fishes while secondary oxytocic injection is conducted on the hemibagrus nemurus female fishes, the female fishes and the male fishes are bred in different spawning ponds, water temperatures in the two ponds are kept to be 25-27 DEG C, breeding is conducted for 10-11 hours, artificial egg stripping is conducted on the mature hemibagrus nemurus female fishes, testes of the hemibagrus pluriradiatus male fishes are cut out, sperm is collected, and dry method artificial insemination is conducted; fertilized eggs are conveyed into a flowing water tank and subjected to artificial incubation and fry breeding in flowing water at the temperature of 25-27 DEG C, and hybridized hemibagrus fry are obtained. The obtained hybridized offsprings are good in body color, high in growth speed, high in temperature adaptability and beneficial to breeding.