![蛋白质](/CN/2015/8/1/images/201580006245.jpg)
基本信息:
- 专利标题: 蛋白质
- 专利标题(英):Protein
- 申请号:CN201580006245.8 申请日:2015-01-30
- 公开(公告)号:CN105992819A 公开(公告)日:2016-10-05
- 发明人: R·H·洛伦森 , S·阿兰特伦德 , I·尼古拉耶夫 , J·亨德里克阿范图埃尔 , B·科普斯
- 申请人: 杜邦营养生物科学有限公司
- 申请人地址: 丹麦哥本哈根
- 专利权人: 杜邦营养生物科学有限公司
- 当前专利权人: 杜邦营养生物科学有限公司
- 当前专利权人地址: 丹麦哥本哈根
- 代理机构: 北京市中咨律师事务所
- 代理人: 凌立; 黄革生
- 优先权: 1401648.9 2014.01.31 GB
- 国际申请: PCT/EP2015/051982 2015.01.30
- 国际公布: WO2015/114112 EN 2015.08.06
- 进入国家日期: 2016-07-28
- 主分类号: C12N9/42
- IPC分类号: C12N9/42 ; C12N15/56 ; C12P19/14 ; C12C5/00 ; C12C7/04 ; A23K20/189 ; A23K10/30 ; A23K10/38
The present inventions relates to a modified GH10 xylanase enzyme or a fragment thereof having xylanase activity wherein said modified GH10 xylanase or fragment thereof has increased thermostability compared with a parent GH10 xylanase enzyme, the parent GH10 xylanase having been modified at, at least, two of the following positions 7, 33, 79, 217 and 298, wherein the numbering is based on the amino acid numbering of FveXyn4 (SEQ ID No. 1). In particular, the modified xylanase enzyme according to the present invention has the following modifications: N7D; T33V; K79Y, K79V, K79F, K79I, K79L or K79M (preferably K79Y, K79F or K79V, more preferably K79Y); A217Q, A217E, A217P, A217D or A217M (preferably A217Q, A217E or A217P, more preferably A217Q); and T298Y, T298F or T298W (preferably T298Y or T298F, more preferably T298Y).
公开/授权文献:
- CN105992819B 蛋白质 公开/授权日:2021-07-13