基本信息:
- 专利标题： 一种重组大肠杆菌菌株及用其制备番茄红素的方法
- 专利标题（英）：Recombinant escherichia coli strain and method for preparing lycopene by adopting same
- 申请号：CN201610292894.5 申请日：2016-05-05
- 公开(公告)号：CN105969709A 公开(公告)日：2016-09-28
- 发明人： 陈浩 , 陈浪 , 周卫强 , 张莎莎 , 胡开蕾 , 晏礼明 , 陶勇 , 刘伟丰
- 申请人： 中国科学院微生物研究所
- 申请人地址： 北京市朝阳区北辰西路1号院3号
- 专利权人： 中国科学院微生物研究所
- 当前专利权人： 中国科学院微生物研究所
- 当前专利权人地址： 北京市朝阳区北辰西路1号院3号
- 代理机构： 北京律和信知识产权代理事务所
- 代理人： 武玉琴; 鲍晓芳
- 生物保藏信息： CGMCC No.12203 20160311
- 主分类号： C12N1/21
- IPC分类号： C12N1/21 ; C12P5/02 ; C12R1/19
The invention provides a method for preparing lycopene by utilizing recombinant escherichia coli through high-density culture and biotransformation, belonging to the field of microbial fermentation. A seed medium and a fermentation medium adopted by the method are media with definite chemical components, and the media are low in cost and contain no organic nitrogen source. According to the method, the lycopene is prepared through a biotransformation method promoting thallus growth and inducing as well as realizing the uncoupling of the transformation period, therefore, the rapid thallus growth is effectively guaranteed, and the preparation time and cost are saved. The fermentation medium and the method provided by the invention are adopted for carrying out high-density culture on escherichia coli, meanwhile, through induction and transformation, the lycopene is prepared by taking glucose as a substrate, the thallus density (OD600nm) and the yield of the lycopene achieve 200 and 3.2g/L fermentation liquor, respectively, and the fermentation period is 36 h.