基本信息:
- 专利标题： 一种基于双聚合酶延伸胸腺嘧啶原位生成铜纳米簇的microRNA检测方法
- 专利标题（英）：MicroRNA detection method based on in-situ generation of Cu-nanoclusters from thymine formed through extension of double polymerases
- 申请号：CN201610167371.8 申请日：2016-03-23
- 公开(公告)号：CN105886610A 公开(公告)日：2016-08-24
- 发明人： 梁汝萍 , 迟宝珠 , 邱建丁
- 申请人： 南昌大学
- 申请人地址： 江西省南昌市红谷滩新区学府大道999号
- 专利权人： 南昌大学
- 当前专利权人： 南昌大学
- 当前专利权人地址： 江西省南昌市红谷滩新区学府大道999号
- 代理机构： 南昌洪达专利事务所
- 代理人： 刘凌峰
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
The invention discloses a microRNA detection method based on in-situ generation of Cu-nanoclusters from thymine formed through extension of double polymerases, belonging to the field of optical biosensing technologies. Based on the co-catalysis of polymerase Klenow fragments and terminal deoxynucleotidyl transferase, when microRNA exists, deoxythymidine triphosphate is arranged and extended to form a long poly-thymine base sequence, and red fluorescence Cu-nanoclusters are generated through in-situ reaction by taking the poly-thymine base sequence as a template, wherein the fluorescence intensity of the Cu-nanoclusters is in direct proportion to the logarithm of the concentration of microRNA, and thus the low-background and high-sensitivity detection for microRNA is realized.