The invention discloses regeneration vegetable oil modified solvent-free self-leveling coating and a preparation method thereof. The coating is composed of a component A and a component B according to a certain ratio. The component A is solvent-free epoxy resin coating, and the component B is epoxy hardener. The component A is prepared from epoxy resin, epoxy modified organic silicon resin, reactive diluent, thickener, a flatting agent, a dispersing agent, a defoaming agent, pigment and padding according to a certain ratio. The preparation method of the regeneration vegetable oil modified solvent-free self-leveling coating comprises the steps that firstly, the raw materials of the component A are weighed according to the ratio; secondly, the epoxy resin and the epoxy modified organic silicon resin are mixed evenly to obtain mixed resin; thirdly, the remaining raw materials are sequentially added into the mixed resin, and after uniform dispersing, grinding is conducted to obtain the component A; fourthly, the component A and the component B are mixed evenly in proportion. The regeneration vegetable oil modified solvent-free self-leveling coating has the advantages of being excellent in crack resistance, crushing resistance, salt tolerance, abrasion resistance and chemical resistance, high in hardness and the like. The operation method is simple, operation is convenient, and the preparation cost is low.