
基本信息:
- 专利标题: 一种棘腹蛙感染弗氏柠檬酸杆菌的PCR快速检测试剂盒及检测方法
- 专利标题(英):PCR (polymerase chain reaction) rapid detection kit and detection method for quasipaa boulengeri infected citrobacter freundii
- 申请号:CN201610292184.2 申请日:2016-05-05
- 公开(公告)号:CN105734163A 公开(公告)日:2016-07-06
- 发明人: 樊汶樵 , 姚昱岑 , 姜玉松 , 徐敬明 , 罗洁 , 吴宝红
- 申请人: 重庆文理学院
- 申请人地址: 重庆市永川区红河大道319号
- 专利权人: 重庆文理学院
- 当前专利权人: 重庆文理学院
- 当前专利权人地址: 重庆市永川区红河大道319号
- 代理机构: 北京一格知识产权代理事务所
- 代理人: 滑春生
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12Q1/10
The invention discloses a PCR (polymerase chain reaction) rapid detection kit and a detection method for quasipaa boulengeri infected citrobacter freundii. The detection kit comprises 1.0mL of a 2*reaction mixed buffer solution, a pair of forward primer and reverse primer, a Taq DNA polymerase, a positive control solution, a negative control solution, and ddH2O. The detection kit disclosed by the invention, when used for detecting the citrobacter freundii of quasipaa boulengeri, not only is feasible for clinical diagnosis, but also has the advantages of fastness, accuracy and strong specificity, and meets requirements of clinical diagnosis, thereby providing a convenient method for detecting the quasipaa boulengeri infected citrobacter freundii.