The invention relates to a method for tomato spotted wilf virus inoculation, and belongs to the field of plant protection.The method includes the following steps that 1, 50 mM of phosphate buffer solution with the pH value of 7.2 is prepared and sterilized; 2, tomatoes are sown, and after four main leaves grow out, tomato plants are used for inoculation; 3, the phosphate buffer solution is added by the ratio of mass: volume=1:5, and susceptible tomato leaves stored at low temperature are ground to obtain homogenate; 4, the homogenate is taken and centrifuged at low temperature and high speed, and supernate is added into a small plastic sprinkling can; 5, after tomato seedlings are soaked in the phosphate buffer solution in an ultrasonic cleaning instrument and oscillated for 10 seconds, virus liquid in the sprinkling can is sprayed on treated tomato seedling leaves; 6, after being cultured for 12 h at the 100% relative humidity in a dark manual climatic box, the virus is taken out and cultured on the conventional conditions.The method has the advantages that the speed of tomato spotted wilf virus inoculation can be increased, the whole-plant tomato leaves are made susceptible, resistance of tomatoes to the virus is fast and effectively verified, and breeding of disease-resistant varieties is accelerated.