基本信息:
- 专利标题： 具有隔离围栏的微流体装置及用它测试生物微目标的方法
- 专利标题（英）：Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
- 申请号：CN201480058444.9 申请日：2014-10-22
- 公开(公告)号：CN105658781A 公开(公告)日：2016-06-08
- 发明人： E·D·霍布斯 , M·P·怀特 , J·坦纳·内维尔 , D·马莱奥 , 史蒂文·W·肖特
- 申请人： 伯克利照明有限公司
- 申请人地址： 美国加利福尼亚州
- 专利权人： 伯克利照明有限公司
- 当前专利权人： 伯克利之光生命科技公司
- 当前专利权人地址： 美国加利福尼亚州
- 代理机构： 隆天知识产权代理有限公司
- 代理人： 金鹏; 周滨
- 优先权： 61/996,969 2013.10.22 US; 62/058,658 2014.10.01 US
- 国际申请： PCT/US2014/061837 2014.10.22
- 国际公布： WO2015/061497 EN 2015.04.30
- 进入国家日期： 2016-04-22
- 主分类号： C12M1/24
- IPC分类号： C12M1/24 ; C12M1/34 ; G01N35/08
A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological microobject can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.
公开/授权文献:
- CN105658781B 具有隔离围栏的微流体装置及用它测试生物微目标的方法 公开/授权日：2019-07-02