
基本信息:
- 专利标题: 一种电化学葡萄糖生物传感器的制备方法及其用于葡萄糖测试的检测方法
- 申请号:CN201610007745.X 申请日:2016-01-07
- 公开(公告)号:CN105548317B 公开(公告)日:2018-07-27
- 发明人: 李永芳 , 薛峰 , 杨中智 , 李长恭 , 祝勇
- 申请人: 河南科技学院
- 申请人地址: 河南省新乡市华兰大道东段河南科技学院化学化工学院
- 专利权人: 河南科技学院
- 当前专利权人: 河南科技学院
- 当前专利权人地址: 河南省新乡市华兰大道东段河南科技学院化学化工学院
- 代理机构: 郑州金成知识产权事务所
- 代理人: 郭增欣
- 主分类号: G01N27/327
- IPC分类号: G01N27/327
The invention relates to a preparation method of an electrochemical glucose biosensor and a detection method for testing glucose. The preparation method includes the steps of dispersing calcium titanate nanometer particles in a chitosan solution to prepare a GOD/CaTiO3/CS mixed solution; modifying the surface of a preprocessed glassy carbon electrode with the prepared GOD/CaTiO3/CS mixed solution to obtain the electrochemical glucose biosensor. The method for detecting a glucose sample through the electrochemical glucose biosensor prepared through the preparation method includes the following steps of firstly, adding the glucose sample to test liquid with the prepared modified electrode as the working electrode, a saturated calomel electrode as the reference electrode and a platinum sheet electrode as the counter electrode; secondly, detecting electrochemical signals of the working electrode through an electrochemical working station. Glucose oxidase is fixed through calcium titanate nanometer particles, and the biology catalytic activity of biological macromolecules can be well kept.
公开/授权文献:
- CN105548317A 一种电化学葡萄糖生物传感器的制备方法及其用于葡萄糖测试的检测方法 公开/授权日:2016-05-04
IPC结构图谱:
G | 物理 |
--G01 | 测量;测试 |
----G01N | 借助于测定材料的化学或物理性质来测试或分析材料 |
------G01N27/00 | 用电、电化学或磁的方法测试或分析材料 |
--------G01N27/02 | .通过测试阻抗 |
----------G01N27/28 | ..电解池部件 |
------------G01N27/30 | ...电极,例如测试电极;半电池 |
--------------G01N27/327 | ....生物化学电极 |