基本信息:
- 专利标题： GI型诺如病毒病毒样颗粒的制备方法及其应用
- 专利标题（英）：Preparing method for GI type norovirus virus-like particles and application thereof
- 申请号：CN201610023370.6 申请日：2016-01-14
- 公开(公告)号：CN105505953A 公开(公告)日：2016-04-20
- 发明人： 张晓文 , 王群 , 郑小龙 , 赵玉然 , 岳志芹 , 房保海 , 梁成珠 , 贾俊涛 , 王宫璞
- 申请人： 山东出入境检验检疫局检验检疫技术中心
- 申请人地址： 山东省青岛市市南区瞿塘峡路70号
- 专利权人： 山东出入境检验检疫局检验检疫技术中心
- 当前专利权人： 山东出入境检验检疫局检验检疫技术中心
- 当前专利权人地址： 山东省青岛市市南区瞿塘峡路70号
- 代理机构： 济南泉城专利商标事务所
- 代理人： 刘帅帅
- 主分类号： C12N15/40
- IPC分类号： C12N15/40 ; C12N15/11 ; C12N15/70 ; C12N7/04 ; C12Q1/70 ; C12Q1/68 ; C12R1/93
The invention discloses a preparing method for virus-like particles containing norovirus GI genes. The virus-like particles are an RNA-protein complex formed by wrapping NV-GI RNA with MS2 bacteriophage encoding coat protein; a primer is designed and artificially synthesized, the norovirus GI gene sequence is amplified by means of the RT-RCR technology and cloned into pNH-MS2his, and pronucleus recombinant expression plasmid pNH-MS2his-NV-GI is constructed. The obtained pNH-MS2his-NV-GI plasmid is converted in expression escherichia coli BL21(DE3) to carry out inducible expression. The virus-like particles can serve as standards and quality control products for detecting RT-PCR, are free of infectivity, safe, reliable and good in stability, and have the advantage of resisting ribonuclease.