基本信息:
- 专利标题： 一种辅助诊断急性缺血性脑卒中的早期生物标志物及其基因芯片和实时荧光定量PCR评价方法
- 专利标题（英）：Early biomarker for auxiliary diagnosis of acute ischemic stroke, and gene chip and real-time fluorescent quantitative PCR (polymerase chain reaction) evaluation methods of early biomarker
- 申请号：CN201510985489.7 申请日：2015-12-25
- 公开(公告)号：CN105441550A 公开(公告)日：2016-03-30
- 发明人： 吕燕妮 , 付龙生 , 陈璿瑛 , 魏筱华 , 温金华 , 欧阳爱军
- 申请人： 南昌大学第一附属医院
- 申请人地址： 江西省南昌市永外正街17号
- 专利权人： 南昌大学第一附属医院
- 当前专利权人： 南昌大学第一附属医院
- 当前专利权人地址： 江西省南昌市永外正街17号
- 代理机构： 江西省专利事务所
- 代理人： 殷勇刚
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12N15/113
The invention discloses an early biomarker for auxiliary diagnosis of acute ischemic stroke, which is microRNA-27a-3p in plasma. The invention also discloses a gene chip evaluation method and a real-time fluorescent quantitative PCR (polymerase chain reaction) evaluation method of the plasma microRNA-27a-3p serving as the early biomarker for auxiliary diagnosis of the acute ischemic stroke. By adopting combination of a gene chip and a real-time quantitative PCR technology, a differential expression of the plasma microRNA-27a-3p is discovered. On the basis of the differential expression, the diagnostic efficiency of the microRNA-27a-3p for the acute ischemic stroke is investigated. An experimental result shows that the expression level of the plasma microRNA-27a-3p in plasma of an acute ischemic stroke patient is significantly lower than that in a healthy person, and a critical value and the sensitivity and specificity of the microRNA-27a-3p for auxiliary diagnosis of the acute ischemic stroke patient are preliminarily determined.