基本信息:
- 专利标题： 一种猪细小病毒环介导等温扩增试剂盒及其应用
- 专利标题（英）：PPV (porcine parvovirus) LAMP (loop-mediated isothermal amplification) kit and application thereof
- 申请号：CN201510869700.9 申请日：2015-11-27
- 公开(公告)号：CN105349702A 公开(公告)日：2016-02-24
- 发明人： 陈忠伟 , 何颖 , 赵武 , 卢冰霞 , 梁家幸 , 秦毅斌 , 段群棚 , 李斌 , 周英宁 , 蒋冬福 , 杨思仪 , 苏乾莲
- 申请人： 广西壮族自治区兽医研究所
- 申请人地址： 广西壮族自治区南宁市西乡塘区友爱北路51号
- 专利权人： 广西壮族自治区兽医研究所
- 当前专利权人： 广西壮族自治区兽医研究所
- 当前专利权人地址： 广西壮族自治区南宁市西乡塘区友爱北路51号
- 代理机构： 广州市红荔专利代理有限公司
- 代理人： 李彦孚; 何承鑫
- 主分类号： C12Q1/70
- IPC分类号： C12Q1/70 ; C12Q1/68 ; C12R1/93
The invention discloses a PPV (porcine parvovirus) loop-mediated isothermal amplification kit and application thereof. The kit comprises LAMP primers, a 2*reaction buffer solution, Bst DNA polymerase, a fluorescent visual detection reagent, ultrapure water and a PPV DNA template, wherein the LAMP primers comprise outer primers F3 and B3, inner primers FIP and BIP, and loop primers LF and LB. The application lies in that the kit is used for detecting PPV lesion tissue samples and carrying out qualitative research on PPV pathogens. Detection and sensitivity detection results prove that the LAMP detection method provided by the invention can monitor the reaction in real time, and quantitatively detect the copy number of PPV, quickly and accurately obtains the detection result, and brings convenience to easy, convenient, quick and reliable PPV detection.