The invention relates to the field of experiment instruments, in particular to a reagent bottle opener which mainly aims at facilitating opening of a reagent bottle cover body. The reagent bottle opener comprises a first panel, a first connecting part and a handle. A first through hole is formed in the first panel, and the diameter of the first through hole is larger than or equal to the outer overall dimension of a reagent bottle inner cover body brim. The first connecting part is connected with one side of the first panel. The connecting end of the handle is connected with the first connecting part, and the holding end of the handle is located in the direction away from the first panel. The first through hole comprises a first half ring close to one side of the first panel and a second half ring close to the other side of the first panel. A first set of prying openings with the prying opening dimensions matched with the outer contour of an inner cover body cylinder with the diameter being the first dimension are oppositely formed in the first half ring and the second half ring. After the inner cover body is pried by being pulled and pushed multiple times by experiment staff, the inner cover body is pried out from the opening of a reagent bottle, and harm to people when the reagent bottle inner cover body is opened is reduced.