基本信息:
- 专利标题： 培养容器和培养方法
- 专利标题（英）：Culture vessel and culture method
- 申请号：CN201480032635.8 申请日：2014-06-05
- 公开(公告)号：CN105308170A 公开(公告)日：2016-02-03
- 发明人： 江尻洋子 , 绫野贤 , 福原直人 , 谷口英树 , 武部贵则
- 申请人： 株式会社可乐丽 , 公立大学法人横滨市立大学
- 申请人地址： 日本冈山县
- 专利权人： 株式会社可乐丽,公立大学法人横滨市立大学
- 当前专利权人： 康宁股份有限公司,公立大学法人横滨市立大学
- 当前专利权人地址： 日本冈山县
- 代理机构： 北京林达刘知识产权代理事务所
- 代理人： 刘新宇; 张会华
- 优先权： 2013-120915 2013.06.07 JP
- 国际申请： PCT/JP2014/002993 2014.06.05
- 国际公布： WO2014/196204 JA 2014.12.11
- 进入国家日期： 2015-12-07
- 主分类号： C12M3/00
- IPC分类号： C12M3/00 ; C12N5/071
A microspace structure for highly efficiently producing spheroids of uniform size and easily replacing and recovering culture medium is designed, and a culture vessel having the designed microspace structure is provided. A plurality of recesses (10) comprising a bottom part (11) and an opening part (12) are arranged in the culture vessel. The bottom part (11) has the shape of either a hemisphere or a truncated cone, and the opening part (12) is constituted by walls having a taper angle of from 1DEGto 20DEG and surrounding an area from the boundary with the bottom part (11) to the end part of the recess (10). In addition, the equivalent diameter at the boundary is from 50 Mum to 2 mm, the depth from the bottom of the bottom part (11) to the end part is 0.6 to 3 times the equivalent diameter, the walls constituting the opening part (12) form a continuous surface with the bottom part (11), and the slope of the continuous surface changes at the boundary.
公开/授权文献:
- CN105308170B 培养容器和培养方法 公开/授权日：2018-04-10
IPC结构图谱:
| C | 化学；冶金 |
| --C12 | 生物化学；啤酒；烈性酒；果汁酒；醋；微生物学；酶学；突变或遗传工程 |
| ----C12M | 酶学或微生物学装置 |
| ------C12M3/00 | 组织、人类、动物或植物细胞或病毒培养装置 |