The invention provides a mink Aleutian disease virus antibody colloidal gold test strip and a manufacturing method thereof and relates to the technical field of colloidal gold test paper strips. The problem that an existing AD detection technology comprises complicated detection steps, consumes time, needs a specialized person to operate and mainly depends on a laboratory during detection is solved. The test strip comprises a PVC base plate, a sample pad, a gold labeled conjugate pad, a nitrocellulose membrane provided with a detection line and a quality control line and a water absorption pad, wherein the sample pad, the gold labeled conjugate pad, the nitrocellulose membrane and the water absorption pad are sequentially pasted on the PVC base plate. The gold labeled conjugate pad is coated with a purified mink Aleutian disease virus cell antigen and colloidal gold coupling marker and a mouse IgG and colloidal gold coupling marker, the detection line on the nitrocellulose membrane is coated with a purified mink Aleutian disease virus cell antigen, and the quality control line on the nitrocellulose membrane is coated with goat anti-mouse IgG. The test strip has the advantages that the test strip is simple and quick to operate, good in specificity and high in sensibility, a detection result is clear and easy to judge, no instruments or equipment is needed.