The invention discloses a flower formation regulator gene LcFLC obtained from litchi chinensis by separation and application thereof, belongs to the field of biological genes. The invention particularly discloses the nucleotide sequence, cloning method and subcellular localization of the gene LcFLC; due to spatio-temporal expression features at different tissue parts of litchi chinensis, the gene LcFLC has the function of retarding the flowering time of plants. According to the gene LcFLC for retarding the flowering time of plants and the application thereof, provided by the invention, the preliminary knowledge about the floral induction mechanism of litchi chinensis is gained for the first time from the perspective of molecular biology, a foundation is laid for regulating the flowering time by overexpression or expression inhibition of target genes in litchi chinensis, and the gene LcFLC has a very important significance to breeding of new litchi chinensis species by utilizing genetic engineering.