The invention discloses a tank limiting device for a mushroom paste sterilizing production line. The tank limiting device for the mushroom paste sterilizing production line comprises a support rotating shaft array composed of support rotating shafts, wherein each support rotating shaft comprises a cylindrical rotating shaft main body; two ends of the rotating shaft main body are each provided with a fixed shaft head; and the rotating shaft main body is provided with a limited support array composed of limited supports. Because of the damping effects of the limited supports, the problem of tank crushing caused by collision between glass tanks is effectively avoided, and the usability and reliability of the mushroom paste sterilizing production line are improved. The tank limiting device for the mushroom paste sterilizing production line is simple in structure, low in cost, remarkable in effect and high in practicability.