基本信息:
- 专利标题： 强冬性白菜型冬油菜SOD酶分子标记及QTL位点
- 专利标题（英）：Strong-winter winter rape (Brassica campestris) SOD enzyme molecular marker and QTL locus
- 申请号：CN201510211290.9 申请日：2015-04-29
- 公开(公告)号：CN105039508A 公开(公告)日：2015-11-11
- 发明人： 孙万仓 , 杨刚 , 曾秀存 , 刘自刚 , 武军艳 , 方彦 , 李学才 , 孔德晶 , 赵艳宁 , 米超
- 申请人： 甘肃农业大学
- 申请人地址： 甘肃省兰州市安宁区营门村1号
- 专利权人： 甘肃农业大学
- 当前专利权人： 甘肃农业大学
- 当前专利权人地址： 甘肃省兰州市安宁区营门村1号
- 代理机构： 甘肃省知识产权事务中心
- 代理人： 孙惠娜
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12N15/11
The invention discloses a strong-winter winter rape (Brassica campestris) SOD enzyme molecular marker and a QTL locus. A screening process includes following steps: (1) performing hybridization between a multi-generation bagged-selfing parent strong-winter super-cold-winder-resistant rape 'Longyou #7' and strong-cold-winder-resistant rape 'Longyou #9' to construct an F2-generation segregation population; (2) extracting leaf DNA of the two parents and the F2-generation segregation population through the CTAB method; (3) performing polymorphic primer screening to the two parent DNA through SSR and InDel primers; (4) obtaining genotype data through the F2-generation segregation population to construct a genetic linkage map and carry out QTL analysis; and (5) obtaining two QTL loca of SOD enzyme of the strong-winter winter rape (Brassica campestris), which are respectively named Qsod.gsau-6A and Qsod.gsau-7A. Four molecular markers, which are linked with the QTL loca of the SOD enzyme of the strong-winter winter rape (Brassica campestris), are respectively named Ra1-F06, Ra2-D04, 8C0108 and Ol12E03.
公开/授权文献:
- CN105039508B 强冬性白菜型冬油菜SOD酶分子标记及QTL位点 公开/授权日：2018-08-31