The invention discloses a rooting agent for raising seedlings of bamboo willows by cutting, and belongs to the technical field of forestry and biology. Every 1000mL of the rooting agent comprises the following raw materials by weight: 50-100mg of an indolebutyric acid, 20-50mg of alpha-sodium naphthalene acetate, 50-100mg of ABT No. 2 rooting powder, 20-50mg of 6-benzyladenine, 2000-3000mg of potassium permanganate, 100-150mL of ethanol of 95%, and water is diluted to 1000mL. The rooting agent is used for directly soaking the bamboo willows, and a treatment manner of semi-lignifying a twig cutting base is applied to cutting, propagation and seedling culture of twigs of the bamboo willows. The rooting agent is used for soaking the twigs of the bamboo willows, then cutting is performed on the soaked twigs, the rooting rate reaches more than 92%, and the survival rate by cutting is obviously increased. The rooting agent disclosed by the invention has the advantages that the raw materials are easy to purchase, the cost is low, the operation is convenient, the dosage is little, the rooting agent takes effect quickly, the effect is good, the rooting agent is non-toxic and harmless, and the rooting agent acts obviously, so that the rooting agent can be extensively popularized and applied.