
基本信息:
- 专利标题: 一种氧化型β-烟酰胺腺嘌呤二核苷酸磷酸的纯化方法
- 专利标题(英):Method for purifying oxidized beta-nicotinamide adenine dinucleotide phosphate
- 申请号:CN201510255144.6 申请日:2015-05-19
- 公开(公告)号:CN104876993A 公开(公告)日:2015-09-02
- 发明人: 傅荣昭 , 戴柱 , 张琦
- 申请人: 邦泰生物工程(深圳)有限公司
- 申请人地址: 广东省深圳市宝安区西乡铁岗工业区4栋(创富厂)2楼
- 专利权人: 邦泰生物工程(深圳)有限公司
- 当前专利权人: 邦泰生物工程(深圳)有限公司
- 当前专利权人地址: 广东省深圳市宝安区西乡铁岗工业区4栋(创富厂)2楼
- 代理机构: 深圳市君胜知识产权代理事务所
- 代理人: 王永文; 刘杰
- 主分类号: C07H19/207
- IPC分类号: C07H19/207 ; C07H1/06
The invention discloses a method for purifying oxidized beta-nicotinamide adenine dinucleotide phosphate. The method for purifying oxidized beta-nicotinamide adenine dinucleotide phosphate concretely comprises the following steps: a, successively carrying out microfiltration and nanofiltration on a pretreated coenzyme II solution by adopting membrane concentration equipment, so that a concentrated crude product solution is obtained; b, regulating the pH value of the obtained crude product solution to 2-4, injecting a sample, loading the sample onto a reversed high performance liquid chromatography preparation column, carrying out gradient elution, and purifying, so that a purified sample solution is obtained; and c, carrying out nanofiltration on the purified sample solution by adopting the membrane concentration equipment, and freeze-drying by adopting a vacuum freeze-drying machine, so that purified coenzyme II is obtained. The coenzyme II prepared by adopting the method disclosed by the invention is high in purity, and the yield is high, so that the method disclosed by the invention has a good market prospect.
公开/授权文献:
- CN104876993B 一种氧化型β-烟酰胺腺嘌呤二核苷酸磷酸的纯化方法 公开/授权日:2017-12-15
IPC结构图谱:
C | 化学;冶金 |
--C07 | 有机化学 |
----C07H | 糖类;及其衍生物;核苷;核苷酸;核酸 |
------C07H19/00 | 含有杂环与糖化物基团共有1个杂环原子的化合物;核苷;单核苷酸类;及其脱水衍生物 |
--------C07H19/02 | .共有氮 |
----------C07H19/04 | ..仅含有氮作为杂环原子的杂环基 |
------------C07H19/16 | ...嘌呤基 |
--------------C07H19/20 | ....有被磷酸或多磷酸酯化的糖化物基 |
----------------C07H19/207 | .....磷酸或多磷酸被另外的羟基化合物酯化,如黄素-腺嘌呤二核苷酸或烟酰胺嘌呤二核苷酸(烟酰胺腺嘌呤核苷酸磷酸入C07H21/02 |