The invention provides a preparation method of glyphosate sodium and a glyphosate sodium preparation. The method comprises the following steps: alkaline materials containing sodium ion and solid glyphosate acid are put into a kneading machine to be reacted, the reaction temperature is 5 to 70 DEG C, the rotate speed of the kneading machine is 10 to 50 rpm, and the reaction time is 10 to 300 min. The invention further provides a glyphosate sodium preparation. The glyphosate sodium preparation comprises the following components in percentage by weight: 20 to 95 percent of the glyphosate sodium prepared through the method, 0 to 25 percent of a surface active agent, and 0 to 75 percent of other fills. The preparation method and the glyphosate sodium preparation have the advantages that the method is simple to operate, the reaction condition is mild, drying heat load is reduced, generated nitrosamine is reduced, antioxidants do not need to be added during the reaction process, the involvement of organic solvent is avoided at the same time, a third type of material does not need to be introduced, the pollution is low, and the cost is low.