A pixel structure, a display substrate and a display device are disclosed. The pixel structure comprises a plurality of first pixels, second pixels and third pixels. The first pixel comprises a first sub-pixel arranged at upper-right part and a second sub-pixel arranged at lower-left part. The second pixel comprises the second sub-pixel arranged at upper-right part and a third sub-pixel arranged at lower-left part. The third pixel comprises the third sub-pixel arranged at upper-right part and the first sub-pixel arranged at lower-left part. The second pixels are arranged at the right and upper sides of the first pixels. The third pixels are arranged at the right and upper sides of the second pixels. The first pixels are arranged at the right and upper sides of the third pixels. According to an embodiment, when an image having an oblique boundary, a transverse boundary and/or a vertical boundary is displayed, the phenomenon of color crispening will not occur because the first sub-pixels, the second sub-pixels and the third sub-pixels are arranged along the boundary of the image in a staggered way.