The invention provides a door frame sealing structure of a vehicle. The door frame sealing structure of the vehicle comprises a door frame sealing strip, an inner door board, a stand column decorative board, a door sealing board and a lateral coaming, wherein the door frame sealing strip is provided with a sealing groove framework; the lateral coaming is arranged in the sealing groove framework; the door frame sealing strip is provided with a trim lip side; a trim lip side sealing groove is defined between the trim lip side and the groove bottom of the sealing groove framework; the stand column decorative board is arranged in the trim lip side sealing groove; a sealing bubble is arranged on the door frame sealing strip and located on the groove side of the sealing groove framework; the sealing bubble is connected with the inner door board in an abutting mode; the inner door board and the door sealing board are connected. The door frame sealing structure of the vehicle has the advantage that the sealing quality is improved by optimizing the structure of the door frame sealing strip.