The invention relates to an animal dorsal root ganglion-spinal cord fixed imaging and compression injury combining device, which comprises a dorsal root ganglion-spinal cord fixed imaging device and a microscopic fluorescent dye injection and spinal cord compression injury integrating device, wherein the animal dorsal root ganglion-spinal cord fixed imaging device (1) comprises a horizontal slide block guide rail (1-1), a fixing component (1-2), a slide rod (1-3) and a spine fixing clamp (1-4), and the microscopic fluorescent dye injection and spinal cord compression injury integrating device (2) comprises a vertical-rotating regulating side rod (2-3), a horizontal regulating component (2-4), a microscopic injection component (2-5) and a spinal cord compression component (2-6). The combining device has the advantages that multiple functions are integrated, the operation is simple and convenient, the switching can be realized at any time, different-degree spinal cord injury animal models can be conveniently manufactured, and after the fluorescent dye microscopic injection, a two-photon microscope is used for dynamically observing the pathological and physiological change process of the dorsal root ganglion-spinal cord injury in real time.