基本信息:
- 专利标题： 一种自内裂解大肠杆菌的裂解酶及其应用
- 专利标题（英）：Lyase of self-cleaving escherichia coli, and applications thereof
- 申请号：CN201510197003.3 申请日：2015-04-23
- 公开(公告)号：CN104762285A 公开(公告)日：2015-07-08
- 发明人： 危宏平 , 杨航 , 余军平
- 申请人： 武汉赛思锐微生物技术有限公司
- 申请人地址： 湖北省武汉市武汉市高新大道666号光谷生物城B7栋
- 专利权人： 武汉赛思锐微生物技术有限公司
- 当前专利权人： 靶抗生物医药科技股份有限公司
- 当前专利权人地址： 美国特拉华州多佛市学院路1012号201室
- 代理机构： 武汉科皓知识产权代理事务所
- 代理人： 汪俊锋
- 主分类号： C12N9/88
- IPC分类号： C12N9/88 ; C12N15/60 ; C12N15/70
The invention discloses a lyase of self-cleaving escherichia coli, and applications thereof. A brandnew lyase ClyN is constructed by adopting gene splicing means, the lyase can result in the self-cleaving of the host escherichia coli after being expressed in the escherichia coli, to release intracellularly expressed ATP and protein. Recombinant ClyN expression plasmid can be duplicated in the escherichia coli BL21(DE3), and express for 15 min under the induction of IPTG to observe the obvious phenomenon of inducing the escherichia coli to self-cleave, and the induced cleaving efficiency achieves 99.8%. When the host bacteria express heterologous enhanced green fluorescent protein (EGFP), EGFP can be released in solution after escherichia coli is cleaved, and the release efficiency is 89% of direct ultrasonic breaking efficiency. Therefore, the ClyN can be used for extracting and release of heterologous protein after expression in the escherichia coli, thus having wide application prospects.
公开/授权文献:
- CN104762285B 一种自内裂解大肠杆菌的裂解酶及其应用 公开/授权日：2017-10-27