![一种快速检测鲜乳中沙门氏菌的方法](/CN/2015/1/39/images/201510195909.jpg)
基本信息:
- 专利标题: 一种快速检测鲜乳中沙门氏菌的方法
- 专利标题(英):Method for fast detecting salmonella in fresh milk
- 申请号:CN201510195909.1 申请日:2015-04-23
- 公开(公告)号:CN104726534A 公开(公告)日:2015-06-24
- 发明人: 王永 , 王法云 , 朱海华 , 王慧 , 周莉 , 章建军 , 邱红玲 , 关炳峰 , 谭静 , 平洋
- 申请人: 河南省商业科学研究所有限责任公司
- 申请人地址: 河南省郑州市文化路87号
- 专利权人: 河南省商业科学研究所有限责任公司
- 当前专利权人: 河南省商业科学研究所有限责任公司
- 当前专利权人地址: 河南省郑州市文化路87号
- 代理机构: 郑州立格知识产权代理有限公司
- 代理人: 涂连梅
- 主分类号: C12Q1/10
- IPC分类号: C12Q1/10 ; G01N33/569 ; C12R1/42
The invention relates to a method for fast detecting salmonella in fresh milk and belongs to the technical field of microbiological detection. The method includes: adding a fresh milk sample into buffer solution to obtain fresh milk fixture, wherein the buffer solution contains reagent for removing protein and fat; filtering the fresh milk mixture with filter paper, and using a micro-pore filter membrane to perform extraction filtration on filtrate; cutting the micro-pore filter membrane into pieces after filtration, and adding tetrathionate broth and phosphate buffer solution for washing and soaking to obtain bacterium suspension; adding salmonella immunomagnetic beads into the bacterium suspension, mixing, oscillating, standing on a magnetic rack for layering, and abandoning supernatant; taking lower-layer solution, adding phosphate buffer solution for washing, standing on the magnetic rack for layering, and abandoning the supernatant to obtain concentrated to-be-detected solution; using an Elisa detecting method to detect the to-be-detected solution. By the method, the total salmonella detecting time is 50 minutes, and detecting time is shortened greatly as compared with a national standard method.
公开/授权文献:
- CN104726534B 一种快速检测鲜乳中沙门氏菌的方法 公开/授权日:2017-04-19