基本信息:
- 专利标题： 一种可稳定自发光的重组载体的构建方法及其应用
- 专利标题（英）：Constructing method of stable self-luminous recombinant vector and application of recombinant vector
- 申请号：CN201510128902.8 申请日：2015-03-24
- 公开(公告)号：CN104711285A 公开(公告)日：2015-06-17
- 发明人： 丁莉萍 , 魏建华 , 王宏芝 , 李瑞芬 , 张杰伟 , 陈亚娟 , 张中保 , 黄义
- 申请人： 北京市农林科学院
- 申请人地址： 北京市海淀区曙光花园中路9号
- 专利权人： 北京市农林科学院
- 当前专利权人： 北京市农林科学院
- 当前专利权人地址： 北京市海淀区曙光花园中路9号
- 代理机构： 北京纪凯知识产权代理有限公司
- 代理人： 关畅; 白艳
- 主分类号： C12N15/82
- IPC分类号： C12N15/82 ; C12N15/54 ; C12N15/53 ; C12N1/21 ; A01H5/00 ; C12R1/19
The invention discloses a constructing method of a stable self-luminous recombinant vector and an application of the recombinant vector. The recombinant vector is obtained by inserting a chloroplast rps7 gene upstream homologous arm, a fatty acid reductase encoding gene, a transferase encoding gene, a luciferase alpha-subunit encoding gene, a luciferase beta-subunit encoding gene, a synthetase encoding gene, a flavin reductase encoding gene and a chloroplast ndhB gene downstream homologous arm into a chloroplast expression vector. Two sequences derived from the poplar (populus davidiana) chloroplast DNA on the recombinant vector are taken as the homologous recombinant fragments and the luminous gene is inserted into a spacer region between the poplar chloroplast genome rps7 gene and ndhB gene in a fixed point, so that the luminous gene is effectively expressed in the poplar chloroplast genome, the self-luminous transgenosis poplar is obtained and the application prospect is wonderful.
公开/授权文献:
- CN104711285B 一种可稳定自发光的重组载体的构建方法及其应用 公开/授权日：2017-10-27