The invention relates to a detection reagent plate for early diagnosis of cerebral injury, and belongs to the technical field of the early diagnosis of cerebral injury. The invention uses an S100 protein as a biochemical criterion for the early diagnosis of cerebral injury; and a colloidal gold labeled antibody aiming at antigen and an immobilized unlabeled antibody are fixed on a one-step detection strip or a detection plate, in order to achieve the goal of one-time detection of acute kidney injury. The reagent plate provided by the invention is simple and convenient, and does not need equipment; the result can be interpreted by naked eyes; detection can be carries out in any place; and each agent is individually packaged, can be stored at room temperature and is easy to save. The test box provided by the invention has the advantages of high sensitivity ad strong singularity, can start the detection in the early stage of cerebral injury, and is applicable to early stage screening and diagnosis of cerebral injury.