
基本信息:
- 专利标题: 基于中药药渣生产漆酶酶液及粉状漆酶的方法
- 专利标题(英):Method for producing laccase liquid and laccase powder based on traditional Chinese medicine dregs
- 申请号:CN201410652136.0 申请日:2014-11-17
- 公开(公告)号:CN104357412A 公开(公告)日:2015-02-18
- 发明人: 岳鹍 , 刘鹏 , 潘志恒 , 安娜 , 侯玮 , 王鹿 , 孙勇民
- 申请人: 天津现代职业技术学院 , 天津市轻工业化学研究所有限公司
- 申请人地址: 天津市河西区灰堆柳林东
- 专利权人: 天津现代职业技术学院,天津市轻工业化学研究所有限公司
- 当前专利权人: 天津现代职业技术学院,天津市轻工业化学研究所有限公司
- 当前专利权人地址: 天津市河西区灰堆柳林东
- 代理机构: 天津创智天诚知识产权代理事务所
- 代理人: 田阳
- 主分类号: C12N9/02
- IPC分类号: C12N9/02 ; C12R1/645
The invention relates to a method for producing laccase liquid and laccase powder based on traditional Chinese medicine dregs. The method comprises the following steps: (1) preparing a PDA solid medium; (2) preparing a seed medium; (3) preparing a fermentation enzyme production seed liquid; (4) preparing a fermentation enzyme production medium; (5) fermenting and producing enzyme; (6) extracting laccase liquid; and (7) carrying out spray drying on the laccase liquid to obtain the laccase powder. The method disclosed by the invention is mild in fermentation condition, simple in process and low in cost, and waste materials are recycled, thus reducing pollution of traditional Chinese medicine dregs on environment; by virtue of active ingredients in the traditional Chinese medicine dregs, fungi is induced to produce enzyme, fermentation time is shortened, the produced laccase is high in activity and good in stability; and the produced laccase can be widely applied to the fields of paper making, printing and dyeing, sewage treatment and the like.
公开/授权文献:
- CN104357412B 基于中药药渣生产漆酶酶液及粉状漆酶的方法 公开/授权日:2018-02-13