The invention discloses a natural lacquer wax emulsion with bacteriostasis activity and a preparation method thereof. The natural lacquer wax emulsion is prepared from lacquer wax, a nonionic surfactant, a cationic surfactant, a co-emulsifier, a pH value regulator, an antioxidant and water through the steps of firstly melting and stirring the lacquer wax, the surfactant and the co-emulsifier, then, slowly adding deionized water, and stirring to emulsify, thereby preparing an oil-in-water type emulsion. The prepared emulsion has good storage stability, centrifugal stability, dilution stability and bacteriostasis performance and can be extensively applied to the industries and fields of fruit and vegetable preservation, material waterproofing, cosmetics, leather and the like. According to the natural lacquer wax emulsion with bacteriostasis activity and the preparation method thereof, the production process is easy to implement, and the raw materials are natural and environment-friendly, so that the natural lacquer wax emulsion is suitable for large-scale popularization.